@article {549236, title = {MHC class II pseudogene and genomic signature of a 32-kb cosmid in the house finch (Carpodacus mexicanus)}, journal = {Genome Research}, volume = {10}, number = {5}, year = {2000}, note = {

Hess, C MGasper, JHoekstra, H EHill, C EEdwards, S VengResearch Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.2000/05/16 09:00Genome Res. 2000 May;10(5):613-23.

}, month = {May}, pages = {613-23}, abstract = {

Large-scale sequencing studies in vertebrates have thus far focused primarily on the genomes of a few model organisms. Birds are of interest to genomics because of their much smaller and highly streamlined genomes compared to mammals. However, large-scale genetic work has been confined almost exclusively to the chicken; we know little about general aspects of genomes in nongame birds. This study examines the organization of a genomic region containing an Mhc class II B gene in a representative of another important lineage of the avian tree, the songbirds (Passeriformes). We used a shotgun sequencing approach to determine the sequence of a 32-kb cosmid insert containing a strongly hybridizing Mhc fragment from house finches (Carpodacus mexicanus). There were a total of three genes found on the cosmid clone, about the gene density expected for the mammalian Mhc: a class II Mhc beta-chain gene (Came-DAB1), a serine-threonine kinase, and a zinc finger motif. Frameshift mutations in both the second and third exons of Came-DAB1 and the unalignability of the gene after the third exon suggest that it is a nonfunctional pseudogene. In addition, the identifiable introns of Came-DAB1 are more than twice as large as those of chickens. Nucleotide diversity in the peptide-binding region of Came-DAB1 (Pi = 0.03) was much lower than polymorphic chicken and other functional Mhc genes but higher than the expected diversity for a neutral locus in birds, perhaps because of hitchhiking on a selected Mhc locus close by. The serine-threonine kinase gene is likely functional, whereas the zinc finger motif is likely nonfunctional. A paucity of long simple-sequence repeats and retroelements is consistent with emerging rules of chicken genomics, and a pictorial analysis of the "genomic signature" of this sequence, the first of its kind for birds, bears strong similarity to mammalian signatures, suggesting common higher-order structures in these homeothermic genomes. The house finch sequence is among a very few of its kind from nonmodel vertebrates and provides insight into the evolution of the avian Mhc and of avian genomes generally.

}, keywords = {*Avian Proteins, Animals, Base Composition, Base Sequence, Birds, Chickens, Cosmids/*genetics, Evolution, Molecular, Genes, MHC Class II/*genetics, Genetic Variation, Molecular Sequence Data, Multigene Family, Phylogeny, Proteins/genetics, Pseudogenes/*genetics, Repetitive Sequences, Nucleic Acid/genetics, Sequence Alignment, Sequence Homology, Nucleic Acid, Songbirds/*genetics}, isbn = {1088-9051 (Print)1088-9051 (Linking)}, author = {Hess, C.M. and Gasper, J. and Hoekstra, H. E. and Hill, C. E. and Edwards, S. V.} }